About this manual. This manual describes the operation of the ÄKTA™FPLC™ system. System description, system maintenance and trouble-shooting are also. filter. Sample pump. Buffer select valve (V6). Injection valve (V1). Reverse . Manual. Flowpath. B1 or B2. BufferValveB. Pump A. Pump B. Common inlet. A2. A1. About this manual This manual describes the operation of ÄKTA purifier: Evaluating the UV response Calculate the UV response ratios in the following.

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ÄKTApurifier User Guide – GE Healthcare Life Sciences

For example, if the pH has been set to 7 and the actual pH is 7. Superloop should be manually filled before the method is started. Type the new value. In a domestic environment, it may cause radio interference, in which case manua, user may be required to take suitable measures. A scouting scheme is now defined.

Two people are required to lift the system. To reduce this, use low concentrations or if possible change to buffer components that only have one pKa. Properties from the System Control menu. The supply voltage for the components in the system and the fraction collector is distributed from the rear of the system. The Instant Run window opens. Examples on peak fractionation parameters are: It’s a community-based project which helps to repair anything.


When the purifir configuration after the UV cell has been changed, for example by changing a component or tubing, the delay volume might change as well. Adding information to the report 1 Click Edit Mode to enable changes in the report.

ÄKTApurifier User Guide – GE Healthcare Life Sciences |

BufferPrep eliminates the time consuming manual buffer preparation and titration wkta needed for every pH change in chromatography. All manual interactions are recorded in the Logbook. A number of pre-defined recipes are available. Distilled water ml Buffer B: Make sure that the upper pressure limit of the system is lower than the maximum allowed pressure of the Superloop. Additional buttons are provided in the toolbar.

Only spare parts that are approved or supplied by GE Healthcare may be used for maintaining or servicing the system. UniNet-1 and UniNet-2, optional cabling installed. More information is provided in the Pump P package. Fixed volume and peak fractionation Fixed volume fractionation can also be combined with peak fractionation during the elution.

As an option, the sample can also be applied using a sample pump, for example Pump P Support Agreement purchased with the instrument: Click Help in the dialog for more information on the options in the Method Wizard. Replace if liquid has penetrated the mixer chamber walls and sealings.


ÄKTApurifier – GE Healthcare Life Sciences

At higher pressures, the glass tube might shatter. Material Color Volume of 10 cm G1, G2 33 cm 0.

The installation test run time is approximately 30 min. The subsequent Sections describe: If used, the Union Luer connector replaces the injection fill port in port 3 of the injection valve.

Wipe the surface purufier with a damp cloth. The new flow rate will be used until the end of the run or until a new flow rate instruction is reached in the method.

Preparation Prepare the injection valve purifie connect Superloop as follows: The parameters are found on the Peak Fractionation page. Click OK at the bottom of the Chromatogram Layout window. Smaller values have a greater effect and a value of infinity has no effect.

The check does not include whether the buffering capacity is large enough over the entire pH range. If the system is turned or the fraction collector removed, the external capillaries and other tubing may become entangled in nearby objects and be pulled from their connections causing leakage.